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BioTechnology QUESTION #10584
Question 1
In a fluidised bed bioreactor, the minimum fluidisation velocity depends on:
  • Only the feed substrate concentration
  • Particle density, particle diameter, and liquid viscosity✔️
  • The volumetric mass transfer coefficient $k_La$ only
  • The impeller tip speed and dissolved oxygen
Correct Answer Explanation
The minimum fluidisation velocity $U_{mf}$ — the superficial liquid velocity needed to just suspend settled particles — depends on: (1) density difference between solid and liquid ($\rho_p - \rho_L$), (2) particle diameter $d_p$, and (3) liquid viscosity $\mu_L$. The Ergun equation or its simplified forms (e.g. Wen and Yu) are used to calculate $U_{mf}$. Fluidised beds are used for immobilised enzyme and cell reactors.