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BioTechnology QUESTION #10617
Question 1
Monoclonal antibodies (mAbs) are produced by hybridoma technology, which involves:
  • Immunising mice and isolating serum containing a mixture of all antibodies produced
  • Fusing antigen-specific B lymphocytes with immortal myeloma cells to create stable, antibody-secreting hybridomas✔️
  • Cloning antibody genes into phage display vectors for in vitro selection
  • Chemical conjugation of multiple antibody fragments to increase avidity
Correct Answer Explanation
Hybridoma technology (Köhler and Milstein, 1975 — Nobel Prize 1984) involves: (1) immunising a mouse with the target antigen, (2) isolating antigen-specific splenic B cells, (3) fusing B cells with myeloma cells (immortal, non-secreting) using PEG, (4) selecting hybridomas in HAT medium (unfused myeloma cells die; unfused B cells die naturally), (5) cloning and screening for the desired antibody specificity. Each hybridoma produces a single, uniform monoclonal antibody.