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Biology QUESTION #11088
Question 1
The deposition of cDNA fragments onto an inert solid surface (such as glass or nylon) for simultaneous gene expression analysis is known as:
  • DNA probes
  • DNA polymerase
  • DNA microarrays✔️
  • DNA sequencing
Correct Answer Explanation
DNA microarrays (gene chips, DNA chips) involve the deposition of thousands of cDNA or oligonucleotide sequences onto a solid support (glass slide, silicon chip) in an ordered array. Fluorescently labelled cDNA from experimental samples hybridises to the array, allowing simultaneous measurement of the expression levels of thousands of genes. This technology revolutionised genomics and allows whole-transcriptome analysis in a single experiment.