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MDCAT Biology QUESTION #11283
Question 1
Which enzyme is used to join DNA fragments during recombinant DNA technology?
  • Restriction endonuclease
  • DNA ligase✔️
  • DNA polymerase
  • Reverse transcriptase
Correct Answer Explanation
DNA ligase catalyzes the formation of phosphodiester bonds between adjacent DNA fragments, 'gluing' them together. Restriction enzymes cut DNA, DNA polymerase synthesizes DNA, and reverse transcriptase synthesizes DNA from an RNA template.