Hexokinase is the first enzyme of glycolysis. It catalyzes the phosphorylation of glucose using ATP: \(\text{Glucose} + \text{ATP} \xrightarrow{\text{hexokinase}} \text{Glucose-6-phosphate} + \text{ADP}\). This irreversible step traps glucose inside the cell. Phosphofructokinase-1 catalyzes the conversion of fructose-6-phosphate to fructose-1,6-bisphosphate.