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Food Science and Technology QUESTION #5966
Question 1
Which test is performed to detect coliform bacteria in milk as an indicator of post-pasteurization contamination?
  • Total Plate Count (TPC)
  • Most Probable Number (MPN) or Petrifilm coliform count✔️
  • Reductase Test (MBRT)
  • Phosphatase Test
Correct Answer Explanation
Coliform bacteria (\(E.\;coli\), \(Klebsiella\), \(Enterobacter\)) should be absent or very low in properly pasteurized milk. Detection uses the MPN method (lauryl sulfate tryptose broth with gas production) or direct plating on selective media (VRBA, Petrifilm CC). Coliforms in pasteurized milk indicate post-pasteurization contamination.