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Food Science and Technology QUESTION #5982
Question 1
In the production of WPC (Whey Protein Concentrate), the MWCO of UF membranes used is typically:
  • \(100\text{–}200\;\text{Da}\) (nanofiltration range)
  • \(5{,}000\text{–}30{,}000\;\text{Da}\) (retains whey proteins; \(\beta\)-Lg MW \(= 18{,}300\;\text{Da}\); \(\alpha\)-La MW \(= 14{,}000\;\text{Da}\))✔️
  • \(100{,}000\;\text{Da}\) (retains only casein micelles)
  • \(1{,}000{,}000\;\text{Da}\) (retains only fat globules)
Correct Answer Explanation
UF membranes with \(5{,}000\text{–}30{,}000\;\text{Da}\) MWCO are used for WPC/WPI production. They retain whey proteins (\(\beta\)-Lg MW \(\approx 18{,}300\;\text{Da}\), \(\alpha\)-La MW \(\approx 14{,}000\;\text{Da}\), BSA \(\approx 67{,}000\;\text{Da}\)) while allowing lactose (\(342\;\text{Da}\)), minerals, and water to permeate.